Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2

Figure 9

Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. . (e) HE histological scores of mouse NP tissues by HE staining. . vs. mice without any injection; ^# vs. IVDD mice injected with harboring shRNA against shRNA NC; ^$ vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; ^$ vs. IVDD mice injected with lentivirus harboring overexpression vector NC; ^@ vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data () were assessed by one-way ANOVA, followed by Tukey’s post hoc test. .

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