5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration

Figure 1

Sodium iodate (NaIO₃)-induced oxidative stress and cell death, which was mitigated by treatment with ferroptosis inhibitors in ARPE-19 cells. (a) Representative images of cells treated with PBS and NaIO₃ (35 mM, 2 h) and stained with H2DCFDA (left) and measured using a microplate reader (right, ).  μm. (b) Ferroptosis induced by NaIO₃ under different concentrations ( per concentration, 20 h), analyzed by Cell the Counting Kit (CCK-8). (c) Effect of different inhibitors on NaIO₃-induced cytotoxicity: 33 μM z-VAD (), 40 μM Nec-1 (), and 2 μM ferrostatin-1 (). (d) Effect of different concentrations of iron-chelator deferoxamine (DFO) on NaIO₃ (35 mM, 20 h)-induced cell death ( per concentration), analyzed by CCK-8 assay. , , and . Abbreviations: H2DCFDA: 2,7-dichlorodihydrofluorescein diacetate; Nec-1: necrostatin-1; PBS: phosphate-buffered saline; z-VAD: z-VAD-FMK.

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