5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration

Figure 4

Inhibition of lipoxygenase-5 (5-LOX) reduced NaIO₃-induced oxidative stress in ARPE-19 cells. (a, b) BODIPY C11 staining lipid peroxidation (, 35 mM NaIO₃,15 h, Zileuton 75 μM). (c) FerroOrange staining for intracellular ferrous ions (75 μM Zileuton, ). (d) Ferroptosis-associated protein xCT detected with western blot analysis (18 mM NaIO₃ for 15 h, ). (e) Oxidative stress-induced DNA damage demonstrated with 8-OHdG staining (, 35 mM NaIO₃, 75 μM Zileuton, 15 h).  μm. Abbreviations: xCT: cystine-glutamate antiporter; 8-OHdG: 8-hydroxy-2-deoxyguanosine; Zil: Zileuton.

(a)

(b)

(c)

(d)

(e)