5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration

Figure 5

NaIO₃ induced mitochondrial damage, which was mitigated with LOX-5 inhibition in ARPE-19 cells. (a) Mitochondrial ROS staining with MitoSOX (NaIO₃ 35 mM,15 h, ). (b) Mitochondrial transmembrane potential detected with JC-1 staining (NaIO₃ 35 mM, 15 h, ). (c) ATP production (NaIO₃ 35 mM, 15 h, ). (d) Representative immunofluorescence staining for mitochondrial protein COX-4 and SDHB (NaIO₃ 35 mM, 15 h, ).  μm. (e) The loss of mitochondrial mass was evaluated with western blot analysis of SDHA. Abbreviations: COX-4: cytochrome c oxidase subunit 4; SDHA: succinate dehydrogenase complex, subunit A, flavoprotein variant; SDHB: succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial.

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