HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Figure 5

(a) The Knodell score (Knodell histological activity index) showed that the necroinflammationin the HBO1 knockdown group was lower than that of CCL4 group. The sections were them incubated with 10% normal goat serum and incubated for 1 h for blocking. Primary antibodies were added and incubated overnight at 4°C. Tissue samples were incubated with secondary antibodies. Then, diaminobenzidine (DAB) color development was carried out; the degree of color development was controlled under optical microscope; the samples were dehydrated with gradient ethanol, transparent in xylene, and sealed with neutral gum. Microscopic observation was then performed. (b) HBO1 could reduce the expression of NLRP3 and NF-κB. Data are showed as . Asterisks indicate significant differences ().

(a)

(b)