Autophagy Ameliorates Reactive Oxygen Species-Induced Platelet Storage Lesions

Figure 3

ROS scavenger reduces stored platelet receptor shedding and apoptosis during platelet storage. PRP was pretreated with or without NAC (5 mM), then stored at room temperature on a rocking agitator with continuous agitation. PRP were harvested and analyzed at designated time points (2 h, 24 h, 48 h, and 96 h). Dot plots show the levels of oxidative stress (a), PS exposure (b), and platelet receptor expression (GPIbα and GPVI) (c) in stored platelets at 2 h, 24 h, 48 h, and 96 h. (d) Representative aggregation traces and summary of aggregation percentage of stored platelets in response to collagen (10 μg/mL) or ADP (20 μM). NS: no significance; ; .

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