Wheel-Running Exercise Protects Ovariectomized Mice from Bone Loss via IFN-γ-Mediated Suppression of the NF-κB and MAPK Pathways

Figure 6

The inhibitory effect of IFN-γ on osteoclastogenesis was time-dependent. BMMs were induced to differentiate into osteoclasts, and IFN-γ was added at the beginning (d0) or after 3 days (d3). (a and b) Representative images of TRAP staining (a) and F-actin cytoskeleton staining (b) and quantitative analysis of osteoclast number per well (Oc. N/well). (c) Representative images of resorption pit assay and quantitative analysis of bone resorption area (the percentage of the resorbed pit area per total area). C: control (with M-CSF only); R: RANKL (with M-CSF and RANKL) (3 replicates, ).

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