Wheel-Running Exercise Protects Ovariectomized Mice from Bone Loss via IFN-γ-Mediated Suppression of the NF-κB and MAPK Pathways

Figure 7

Anti-CD119 antibody counteracted the inhibitory effect of IFN-γ on osteoclast differentiation. BMMs were differentiated into osteoclasts with IFN-γ (10 ng/mL) and anti-CD119 antibody (5 ng/mL). (a) Relative expression of osteoclast marker genes after 3 days of differentiation. (b and c) Representative images of TRAP staining (b) and F-actin cytoskeleton staining (c) and quantitative analysis of osteoclast number per well (Oc. N/well). (d) The expression and quantitative analyses of MMP-9, CTSK, and NFATc1 in nuclear extracts of BMMs by western blot performed after 6 days. C: control (with M-CSF only); R: RANKL (with M-CSF and RANKL) (3 replicates, ).

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