Research Article
Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats
Figure 7
Effects of curcumin on DHT-induced ER stress in ovarian GCs mediated via increasing of PI3K/AKT phosphorylation. (a, b) Western blot results showing the changes of the p-PI3K, PI3K, p-AKT, and AKT levels in GCs treated with DHT and 740-YP (a PI3K activator). (c, d) The p-IRE1α expression level in GCs treated with DHT, 740-YP, and curcumin by immunofluorescence staining (60x). (e, f) Changes in ER stress-related protein p-IRE1α, IRE1α, XBP1, GRP-78, and CHOP levels in GCs treated with DHT and 740-YP as detected by western blot. Three independent experiments were performed with similar results. Data are shown as the . and vs. DMSO group; ## vs. DHT group.
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