Curcumin Inhibits Hyperandrogen-Induced IRE1<i>α</i>-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats

<div>Curcumin protects hyperandrogen-induced apoptosis in ovarian GCs in PCOS rats by inhibiting the IRE1<i>α</i>-XBP1 pathway and activating the PI3K/AKT signal. DHT/TM-induced GCs were treated with curcumin, 4u8c, LY294002, and 740-YP with or without DHT. (a) GCs were incubated with Annexin V-FITC and PI. The cells were imaged for apoptosis detection using a FV3000 Olympus microscope. (b, c) Apoptosis protein expression of cleaved-caspase-3 was analyzed by immunofluorescence staining (60x) and quantified using ImageJ software. (d, e) The expression of casapase-12, caspase-9, and Bax was assessed by the western blot assay. Three independent experiments were performed with similar results. 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Oxidative Medicine and Cellular Longevity

fig8

Figure 8

Figure 8: Curcumin Inhibits Hyperandrogen-Induced IRE1<i>α</i>-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats 

Figure 8 | Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats 