Research Article

Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats

Table 2

Reaction conditions of RT-qPCR.

Stage 1PredegenerationRep: 195°C30 sec
Stage 2Circular reactionReps: 4095°C10 sec
60°C30 sec
Stage 3Melting curveRep: 195°C15 sec
60°C60 sec
95°C15 sec