Bovine Lactoferrin Induces Cell Death in Human Prostate Cancer Cells

Figure 5

DU-145 cell treatment with apo- and holo-bLf induces oxidative stress and activates caspases 3/7. Cells were treated with 2, 4, 8, and 16 mg/mL of apo- and holo-bLf for 24 and 48 h and analyzed in high-throughput system Operetta. (a) Oxidative stress with apo-bLf treatment through dihydroethidium- (DHE-) labeled cell. (b) Oxidative stress with holo-bLf treatment through dihydroethidium- (DHE-) labeled cell. (c) Caspases 3/7 activation with apo-bLf treatment through CellEvent Caspase 3/7 Green Detection Reagent. (d) Caspases 3/7 activation with holo-bLf treatment through CellEvent Caspase 3/7 Green Detection Reagent. These graphics represent one distinct experiment with replicates. The experiment is expressed as . Significant differences between untreated and treated cells were compared by the one-way ANOVA test, with Bonferroni post hoc test (; ; ; ).

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