Research Article
Four-Octyl Itaconate Protects Chondrocytes against H2O2-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating Nrf2 Signaling
Figure 1
OI activated Nrf2 signaling in primary murine chondrocytes. (a) The chemical structure of OI. Primary murine chondrocytes were added with OI (25 μM) or vehicle control (“Veh”). (b) Coimmunoprecipitation (“co-IP”) assay was employed to examine the binding of Keap1 and Nrf2. The expressions of the indicated proteins in total cellular lysates (c) and nuclear lysates (d) were determined by western blotting. (e, f) Protein and mRNA expressions of the listed genes were investigated by western blotting analyses and qPCR; (g) relative ARE luciferase activity was detected. Primary murine chondrocytes were added with MG-132 (10 μM, 24 h) and cycloheximide (CHX, 100 μg/ml, 12 h), and OI (25 μM, 4 h) was given subsequently. (h, i) Nrf2 and tubulin protein expressions in total cellular lysates were examined. The values are represented as . vs. “Veh” group.
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