Nanoscale Modification of Titanium Implants Improves Behaviors of Bone Mesenchymal Stem Cells and Osteogenesis In Vivo

Figure 4

(a–c) Fluorescence microscopy images of rBMSCs attached to SLA (a), h1SLA (b), and h2SLA (c) surfaces stained by AO. (d) Quantitative analysis of cell attachment. (e–g) Fluorescence microscopy images of rBMSCs spreading on SLA (e), h1SLA (f), and h2SLA (g) surfaces. Orange red: rhodamine-phalloidin. Blue: DAPI. (h) Quantitative analysis of spreading areas. (i–k) Fluorescence microscopy images of Mki67 expression on SLA (i), h1SLA (j), and h2SLA (k) surfaces at 3 d. Green: Mki67. (l) Quantitative analysis of fluorescence intensity ().