Luteolin Alleviates Epithelial-Mesenchymal Transformation Induced by Oxidative Injury in ARPE-19 Cell via Nrf2 and AKT/GSK-3β Pathway

Figure 7

Knockdown of Nrf2 abolished the inhibitions of luteolin on H₂O₂-induced EMT and activation of the AKT/GSK-3β pathway. APRE-19 cells were treated with H₂O₂ and luteolin at 40 μM. (a) Transwell migration for detecting the number of migrating cells. Data are expressed as ().  μm. ^※※ versus normal control; versus H₂O₂; ^## versus H₂O₂. Western blotting to evaluate the expression of α-SMA, vimentin, ZO-1, and E-cadherin (b, d–g) and AKT, p-AKT, GSK-3β, and p-GSK-3β (c, h, i). Data are expressed as (). ^※※ versus normal control; and versus H₂O₂; ^## and ^# versus H₂O₂.

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