Energy-Stress-Mediated AMPK Activation Promotes GPX4-Dependent Ferroptosis through the JAK2/STAT3/P53 Axis in Renal Cancer

Figure 1

Energy stress promotes ferroptotic cell death in renal cancer cells. (a) Cell death measurement shows 0-Glu promotes erastin-induced cell death. (b) TEM was used to observe morphological changes of cellular ultrastructure. Cells in 0-Glu and erastin treatment group present aberrant mitochondria. Black arrows indicate mitochondria. Scale bars: 1 μm. Independent experiments were repeated three times and representative data were shown. (c–e) 0-Glu and erastin treatment-induced GSH deletion, MDA generation, and cellular iron elevation are rescued by Fer-1. Glu: glucose concentration of RPMI 1640 medium. 0-Glu: 0 mM glucose. Data shown represent from at least three independent experiments. Comparisons were performed using Student’s -test. Fer-1: ferrostatin-1; ns: not significant. .

(a)

(b)

(c)

(d)

(e)