Energy-Stress-Mediated AMPK Activation Promotes GPX4-Dependent Ferroptosis through the JAK2/STAT3/P53 Axis in Renal Cancer

Figure 3

GPX4 overexpression alleviates energy-stress-mediated ferroptotic cell death. (a) VENN diagram shows GPX4 as a ferroptosis-related differentially expressed genes. (b and c) PPI network and coexpression analysis shows the correlation between AMPK and GPX4. (d) Western blotting assay presents a decrease of GPX4 expression after glucose-starvation. (e) Western blotting assay verifies the effect of GPX4 overexpression. (f) Cell death measurement in cells treated with indicated treatments. Independent experiments were repeated three times, and representative data were shown. (g–i) The measurement of GSH, MDA, and cellular iron in cells treated with oeGPX4 or Fer-1 after o-Glu and erastin treatment. Data shown represent from at least three independent experiments. Comparisons were performed using Student’s -test and one-way ANOVA. oeNC: overexpression negative control; Fer-1: ferrostatin-1. and .

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