Mucin 1 Inhibits Ferroptosis and Sensitizes Vitamin E to Alleviate Sepsis-Induced Acute Lung Injury through GSK3β/Keap1-Nrf2-GPX4 Pathway

Figure 2

Ferroptosis occurred in lung tissues and alveolar epithelial cells of sepsis. (a–e) Western blot assay was used to evaluate the expression levels of ferroptosis-related genes; (f–i) expression levels of redox products in the lung tissues of sepsis mice; (j–n) effects of different concentrations of LPS on the expression levels of ferroptosis-related genes; (o–s) effects of different time points of LPS on the expression levels of ferroptosis-related genes; (t, u) flow cytometry was used to detect the levels of lipid peroxides in MLE-12 cells; (v) TEM assessment of the effects of LPS on ferroptosis-related organelle morphology. All data were expressed in the form of , , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)

(r)

(s)

(t)

(u)

(v)