Mucin 1 Inhibits Ferroptosis and Sensitizes Vitamin E to Alleviate Sepsis-Induced Acute Lung Injury through GSK3β/Keap1-Nrf2-GPX4 Pathway

Figure 3

Fer-1 inhibited the occurrence of ferroptosis and alleviated lung injury in lung tissues and alveolar epithelial cells of sepsis. (a, b) Western blot assay was performed to evaluate the effect of Fer-1 on the expression level of GPX4 in lung tissues of septic mice. (c, d) Western blot assay was performed to evaluate the effect of Fer-1 on GPX4 expression in MLE-12 cells. (e–h) Effects of Fer-1 on the expression level of redox products in lung tissues of septic mice. (i, j) Flow cytometry was used to detect the effects of Fer-1 on lipid peroxides in MLE-12 cells. (k) HE staining results in each group. (l) Lung injury score in each group. (m) Wet-dry weight ratio of lung tissues in each group. (n–q) Levels of TNF-α, IL-1β, IL-6, and IL-10 in sera of each group. (r–u) Levels of TNF-α, IL-1β, IL-6, and IL-10 in BALF of each group. All data were expressed in the form of ; , , and .

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