T Lymphocyte-Derived Exosomes Transport MEK1/2 and ERK1/2 and Induce NOX4-Dependent Oxidative Stress in Cardiac Microvascular Endothelial Cells

Figure 6

T cell-derived exosomes upregulate NOX4 in cMVECs through ERK1/2 and MEK1/2 pathway. This panel shows representative microphotographs of immunoblots and changes in levels of selected proteins in cMVECs in response to CD-4 exosomes in presence of ERK1/2 inhibitor (FR180204), MEK1/2 inhibitor (U0126), and NOX inhibitor (GKT136901). Cells were stimulated with exosomes for 16 h, . Protein levels were normalized to beta-tubulin, and phosphorylated proteins were additionally normalized to their respective nonphosphorylated forms. , , and calculated by one-way ANOVA followed by Fisher’s LSD post hoc test versus control group or for indicated groups.