T Lymphocyte-Derived Exosomes Transport MEK1/2 and ERK1/2 and Induce NOX4-Dependent Oxidative Stress in Cardiac Microvascular Endothelial Cells

Figure 7

Oxidative stress caused by T cell-derived exosomes is mediated by NOX4. Panel (a) shows densitometry and representative immunoblots of cMVECs incubated with anti-Nox4 siRNA for 36 h, . Panel (b) shows changes in total ROS, mitochondrial ROS, and NO levels in cMVECs transfected with anti-Nox4 siRNA for 24 h and stimulated with CD4-exosomes for another 16 h, . ns- and calculated by Student’s -test in (a) and one-way ANOVA followed by Fisher’s LSD post hoc test versus indicated groups in (b).

(a)

(b)