STAT6/VDR Axis Mitigates Lung Inflammatory Injury by Promoting Nrf2 Signaling Pathway

Figure 5

The interaction of STAT6 and VDR. (a) Immunofluorescence staining of VDR in the cells with or without siSTAT6 20 nM 48 h transfection and IL4 (20 ng/ml, 24 h) treatment. Nuclei were stained with DAPI (blue). (b) The protein expression of STAT6 and VDR in the nucleus and cytoplasm after transfection of VDR or STAT6 plasmid in BEAS-2B cells. (c) The protein complex of STAT6-VDR in BEAS-2B cells was assayed by immunoprecipitation to analyze the association between STAT6 and VDR at the endogenous level (and indicate the target bind). (d) HEK293T cells overexpressed different combinations of Flag-STAT6 and VDR as indicated. Exogenous STAT6 interacted with VDR was assayed by immunoprecipitation with Flag beads. (e) HEK293T cells were overexpressed with indicated plasmids (Flag-STAT6 and VDR), and the interaction between VDR and STAT6 was detected by immunoprecipitation using agarose beads along with VDR antibodies.

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