Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE/LDLR Pathway in Influenza A-Induced Acute Lung Injury

Figure 7

The effect of re-ApoE on the polarization of BMDMs and melatonin receptors. (a) Quantitative RT-PCR measurements of the relative mRNA levels of MT1 and MT2 in wild-type (WT) BMDMs infected by influenza A (H3N2) (, 12 h) with pretreatments of recombinant ApoE2, ApoE3, and ApoE4 (10 μg/ml, 3 h before H3N2 infection). (b) Western blot images of the expression of ApoE, MT-1/2, iNOS, and Arg1 to β-actin in WT BMDMs. (c) Representative immunofluorescence images of F4/80 (red), iNOS (green), and Arg1 (green) expression in WT BMDMs (original magnification × 400). (d, e) Quantitative RT-PCR measurements of the relative mRNA levels of TNF-α, MCP1, CD86, Arg1, Fizz1, and CD206 in WT BMDMs. Data expressed as (). , , and compared with influenza A- (H3N2-) infected BMDMs.

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