Sitagliptin Alleviates Radiation-Induced Intestinal Injury by Activating NRF2-Antioxidant Axis, Mitigating NLRP3 Inf--lammasome Activation, and Reversing Gut Microbiota Disorder

Figure 3

Sitagliptin prevents radiation-induced apoptosis and DNA damage in intestinal epithelial cells. (a, b) The effect of Sitagliptin on apoptosis in radiation-induced intestinal damage was assessed by TUNEL staining. Scale bars, 100 μm, . (c, d) Representative immunofluorescence images and quantification of C-caspase3 in the small intestinal tissues. Scale bars, 100 μm, . (e, f) Representative images and quantification of γ-H2AX IHC staining in small intestinal tissues. Scale bars, 100 μm, . (g) The expression of γ-H2AX in HIEC-6 cells was examined by western blotting. (h) The formation of γ-H2AX foci was evaluated by immunofluorescence in HIEC-6 cells. Scale bars, 10 μm.

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