Research Article
Fetal Zone Steroids Show Discrete Effects on Hyperoxia-Induced Attenuation of Migration in Cultured Oligodendrocyte Progenitor Cells
Figure 3
Impact of FZS on the migration of OPCs. (a) Representative images of migration (wound healing) assay under normal oxygen conditions (21% O2) and post-24-hour 80% O2 with respective steroid treatments are displayed. Graph represents the combined data from five independent experiments. Scale bar represents 2.5 mm. Western blot analysis of OLN93 cells with (b) cofilin and P-cofilin, (c) LIMK and P-LIMK, and (d) TESK and chronophin antibody at normal (21% O2) conditions and post-24-hour 80% O2 with respective steroid treatments. Data are representative of at least three independent experiments. Bars and error represent of replicate measurements. Labels indicate statistically significant differences in comparison to normoxic control (), in comparison to hyperoxic control (#), and between normoxic and hyperoxic treatments within the same group (§). Single signs represent a value < 0.05, double signs represent , triple signs represent , and quadruple signs represent (Student’s -test).
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