Fetal Zone Steroids Show Discrete Effects on Hyperoxia-Induced Attenuation of Migration in Cultured Oligodendrocyte Progenitor Cells

Figure 6

Adiol+E2 shows regulation of different pathways under hyperoxia and normoxia. (a) Venn diagram of significantly altered proteins (normalized to the normoxic control and hyperoxic control as depicted above each bubble) in OLN93 cells post different treatments. Cutoff value < 0.05. Abbreviations: H_Co: hyperoxic control; H_AD: hyperoxic adiol treatment; H_AD+E2: hyperoxic adiol+E2 cotreatment; N_Co: normoxic control. The “/” mark represents “in comparison to.” (b) Canonical pathway analysis of significantly enriched proteins in the OLN93 cells post cotreatment of adiol+E2 in comparison to normoxic and hyperoxic controls using IPA. Heat-map representation of enriched pathways upon adiol+E2 treatment under hyperoxic condition sorted by -score. Higher negative -score is indicated in blue, and higher positive -score values are indicated in red. Cutoff value < 0.05 (Fisher’s exact test). No enrichment was obtained in the H_AD+E2 vs. H_Ctrl comparison and therefore has not been depicted. Complete heat map is provided as supplementary figure S3. (c) Heat-map representation of cell migration-related proteins that were dysregulated in the different steroid treatment groups post-24-hour 80% O₂ treatment in comparison to normoxic and hyperoxic controls. Protein abundance ratios are depicted with a color scale (downregulated proteins are indicated in blue, intermediate in grey, and upregulated proteins in red). Proteins are sorted according to Gene Ontology (biological process). Dark outlined cells represent the significantly altered proteins () in each group. (d) Canonical pathway analysis of significantly enriched proteins in the OLN93 cells post adiol+E2 cotreatment under normoxic condition in comparison to normoxic control using IPA. Heat-map representation of enriched pathways sorted by -score. Pathways with a -score value of >1 or <-1 are shown. Negative and positive -score values are indicated in blue and red, respectively. Cutoff value < 0.05 (Fisher’s exact test). All data are representative of five independent experiments.

(a)

(b)

(c)

(d)