Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation

Figure 5

Knockdown of SNHG1 and UBL7-AS1 decreases proliferation of U138MG cells. Expression levels of glioblastoma associated lncRNAs correlate with the expression of cell cycle genes. (a) Vulcano correlation plot showing the expression cell-cycle genes positively and negatively correlated with SNHG1 in dataset GSE7696. (b and c) Scatter plots for log expression of lncRNA expression (x-axis) versus log expression of the most positively correlated cell-cycle gene – MAD2L1 in dataset GSE50161 (d) and GSE4290 (e). (d) Vulcano correlation plot showing the expression cell-cycle genes positively and negatively correlated with UBL7-AS1 in dataset GSE7696. (e and f) Scatter plots for log expression of lncRNA expression (x-axis) versus log expression of the most positively correlated cell-cycle gene – CCNB2 in dataset GSE50161 (g) and GSE4290 (h). (g and h) Real-time PCR analysis of the expression of SNHG1 (a) and UBL7-AS1 (b) in U138MG cells transfected with siRNA to SNHG1, UBL7-AS1, or both. (i) Western blotting analysis of the expression of MAD2L1 and CCNB2 in U138MG cells transfected with siRNAs to SNHG1, UBL7-AS1 or both. (j) Cell proliferation analysis in U138MG cells transfected with siRNAs to SNHG1, UBL7-AS1, or both using the CyQUANT assay. (k) Immunostaining for Ki67 in U138MG cells transfected with siRNAs to SNHG1, UBL7-AS1, or both. (l) Quantification of the results in (e). P-values were calculated using one-way ANOVA where: p <0.05, p <0.01, p <0.001, p <0.0001.

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