Mulberrin Confers Protection against Doxorubicin-Induced Cardiotoxicity via Regulating AKT Signaling Pathways in Mice

Figure 5

Mulberrin attenuated the expression of inflammatory cytokines in DOX-treated mice. The mRNA levels of inflammatory factors (a) were also quantified. The protein expression of TNF-α (b) and IL-6 (c) were detected by ELISA. The phosphorylation of NF-κB and IκBα (d) was detected in the indicated groups. The protein expression of Bax and Bcl-2 (e) was detected by western blotting. Myocardial apoptosis were evaluated by the TUNEL staining (f), caspase 3 activity (g), and DNA fragmentation test (h). Data are presented as . for each group. compared with the saline group; ^# compared with DOX alone.

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