The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway

Figure 6

RBP4 can promote VSMCs proliferation and migration under high glucose condition, which can be inhibited by vitamin D. (a) The proliferation ability of VSMCs was measured by MTT, scratch wound-healing, and Transwell migration assay ( for the RCMV-RBP4 group compared to the NC group, ^# for siRNA-RBP4-, AG490-, and vitamin D-treated groups compared to RCMV-RBP4). (b) VSMC migration ability was measured by Transwell assays. (c) VSMC migration ability was measured by scratch wound assays. (d) Flow cytometry was applied to detect the cell cycle of VSMCs. (e) Flow cytometry was applied to detect the apoptosis rate of VSMCs ( for the RCMV-RBP4 group compared to the NC group, ^# for siRNA-RBP4-, AG490-, and vitamin D-treated groups compared to RCMV-RBP4).

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