Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice

Figure 1

Colchicine inhibits carrageenan-induced thrombosis in mouse tail. (a) Experimental design: BALB/c mice in 6 groups (6/group) received the following treatment; negative control group (NC) and control group (Control): i.g. administration of PBS; Tig-1 and Col-1 (prevention groups): i.g. administration of ticagrelor (40 mg/day/kg bodyweight, Tig-1 group) or colchicine (0.05 mg/day/kg bodyweight, Col-1 group) for 4 days; Tig-2 and Col-2 (treatment groups): i.g. administration of ticagrelor (Tig-2 group) or colchicine (Col-2 group) for the last 2 days of treatment. Mice were i.p. injected with carrageenan solution (50 mg/kg bodyweight) on day 2, except for the NC group. Two days after carrageenan injection, mouse tail samples were collected. (b, c) The tail of each mouse was photographed, and the representative pictures were shown (b); the thrombosis rate (the ratio of tail length with thrombus to whole tail length) was calculated (c). (d, e) the cross sections of tail at different tail locations were conducted HE staining (d) and at the location of 3 cm were conducted Masson staining (e). The blue dotted line represents the outline of the blood vessel, and the red dotted line represents the intravenous thrombus. ND: not detected; vs. NC group; ^### vs. control group ().

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