Vaspin Attenuates Atrial Abnormalities by Promoting ULK1/FUNDC1-Mediated Mitophagy

Figure 4

Vaspin alleviates Ang-II-induced mitochondrial dysfunction in HL-1 cells. (a–b) Representative images and quantification of reactive oxygen species (ROS) accumulation using DCFH-DA staining, . (c–d) Representative images and quantification of mitochondrial oxidative stress using MitoSOX staining, . (e–f) Representative images and quantification of TMRM staining, . (g–h) Representative images and quantification of JC-1 staining, , fields/group, data from three independent experiments (panels a–h). (i–j) OXPHOS in Ang-II-stimulated HL-1 cells in the presence or absence of vaspin treatment, samples/group (panels i–j). Data are presented as the , vs. control group, vs. Ang-II group.

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