Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway

Figure 4

Human pulmonary artery smooth muscle cells (HPASMCs) were treated using 0.01 crude drug g/ml TRQ, 50 μM SKF96365, 60 nM cysteine, and/or 200 ng/ml CX3CL1 and undergoing hypoxia (2% O₂) for 24 h. (a) Protein expression of TRPC1 was detected by immunofluorescence. (b) Relative TRPC1 was analyzed (to normal) and vs. normal group. ^# and ^## vs. hypoxia group. . (c) The expression of TRCP1, CX3CR1, HIF-1α, NF-κBp65, and p-NF-κBp65 was measured using western blotting. (d) The expression of TRCP1, CX3CR1, HIF-1α, NF-κBp65, and p-NF-κBp65 was analyzed from blots. and vs. normal group. ^# and ^## vs. hypoxia group. . (e) The level of NF-κBp65 in cell supernatant was assayed using the ELISA kit. and vs. normal group. ^# and ^## vs. hypoxia group. .

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