Gpx3 and Egr1 Are Involved in Regulating the Differentiation Fate of Cardiac Fibroblasts under Pressure Overload

Figure 3

Simulation of the developmental trajectory of CFs and analysis of gene expression patterns inferred by Monocle2. (a) Pseudotime trajectory analysis of fibroblasts, using significantly regulated genes between C1 and C5. (b) Time series inference of cell trajectories. The color gradient indicates pseudotime progression. (c) Cell trajectories were renamed according to three main branches. (d) Cell trajectories of each group. Pie chart showing the proportion of cells in each branch of each group. (e) GO biological process terms enriched in each gene cluster. (f) Heat map hierarchical clustering showing differentially expressed genes together with the pseudotime curve. Color key from green to purple indicates relative expression levels from low to high. (g) Gene network displaying the top 20 hub genes from each cluster. (h, i) Expression of representative profibrotic genes and antioxidative stress genes in each branch.