The Effects of In Utero Fetal Hypoxia and Creatine Treatment on Mitochondrial Function in the Late Gestation Fetal Sheep Brain

Table 2

White matter mRNA gene analysis. Data expressed relative to the geomean of housekeeping genes (RPS16, RPL32, and OAZ1) and shown relative to saline-control fetuses. Saline-control: , creatine-control: , saline-UCO: , and creatine-UCO: . All data are expressed as , analyzed by two-way ANOVA and Tukey’s multiple comparisons test. Bolded text indicates statistically significant result ().

Subcortical white matter		Control		UCO		Statistics
		Saline	Creatine	Saline	Creatine
Cerebral injury markers	CASP3					0.68	0.95	0.33
	BAK					0.49	0.42	0.64
	BAX					0.56	0.64	0.14
	BCL2					0.21	0.55	0.93
	ANGTP2					0.49	0.39	0.02
	HIF1α					0.52	0.50	0.27
	BAX:BCL					0.87	0.99	0.95
Mito genes	BECN1					0.75	0.49	0.05
	CRLS1					0.73	0.88	0.81
	COX					0.47	0.69	0.02
Mito density	TRMT11:B2M					0.87	0.99	0.95
Mitogenesis	PCG1α					0.50	0.43	0.08
	TFAM					0.72	0.59	0.77
Fission	DNML1					0.39	0.18	0.06
	FIS1					0.09	0.40	0.30
	MFF					0.51	0.21	0.33
Fusion	OPA1	1.00 ± 0.12				0.39	0.35	0.13
	MFN1					0.09	0.14	0.47
	MFN2					0.35	0.58	0.31
	MIEF1					0.90	0.72	0.19
Energy metabolism	SIRT1					0.38	0.42	0.63
	SIRT3					0.51	0.88	0.44
	ERRα					0.37	0.41	0.13
	PRKAA1					0.84	0.48	0.20
	PRKAA2					0.18	0.49	0.19
Creatine metabolism	SLC6A8					0.87	0.68	0.46
	CKMT					0.60	0.52	0.06