A Novel Method for Identifying Parkin Binding Agents in Complex Preparations of Herbal Medicines

Figure 3

Determination of a mixed reference solution using the parkin-based fishing method. (a) LC/MS directly assayed HPLC chromatograms of a mixed reference solution. (b) HPLC chromatograms of a mixed reference solution for ultrafiltrates derived from active (black line) and denatured parkin (red line). Peaks R1 and R3 exhibited area enhancement than the controls. ST: DL-selenomethionine; AC: amoxicillin; DTT: DL-dithiothreitol; FB: fenofibrate.