A Novel Method for Identifying Parkin Binding Agents in Complex Preparations of Herbal Medicines

Figure 4

Fishing of parkin-targeted constituents from SFR extracts. HPLC chromatograms of screened SFR extract ((a, b): (a) 0–70 min; (b) 70–140 min) showing four peaks (K1–K4) that were significantly enhanced by specific parkin binding (black line) against controls containing denatured parkin (red line).

(a)

(b)