Isorhapontigenin Modulates SOX9/TOLLIP Expression to Attenuate Cell Apoptosis and Oxidative Stress in Paraquat-Induced Acute Kidney Injury

Figure 4

SRY-box transcription factor 9 (SOX9) and Toll-interacting protein (TOLLIP) participate in the pharmacological effects of isorhapontigenin (ISO). (a, b) Verification of adenovirus- (Ad-) mediated knockdown of SOX9 in NRK-52E cells using Western blot and quantitative real-time PCR (qPCR). Forty-eight hours after Ad transduction, cells were incubated with 300 μM PQ for 24 h. (c, d) Flow cytometry was performed to determine the cell apoptosis. Levels of (e) malondialdehyde (MDA) and (f) lactate dehydrogenase (LDH) were measured using commercially available kits. TOLLIP expression was determined at the (g) protein and (h) mRNA levels using Western blot and qPCR. Error bars represent standard deviations. values < 0.05, values < 0.01, and values < 0.001.

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