Glutamine Transporter SLC1A5 Regulates Ionizing Radiation-Derived Oxidative Damage and Ferroptosis

Figure 5

Glutamine transporter SLC1A5 affected ferroptosis response in liver tumor cell lines. (a) The knockdown efficiency of SLC1A5 in Huh7 cells was determined by qPCR. (b) In shSLC1A5 cells, the results of CCK8 demonstrated a significant reduction in tumor cell proliferation. (c) After RSL3 stimulation (2.5 μM for 6 hours), the level of cell viability in shSLC1A5 Huh7 cells was much lower than it was in shControl cells. (d) In shSLC1A5 cells, the MFI of LPO increased substantially, and the elevated MFI of LPO was reversed by a ferroptosis inhibitor. (e) After Erastin stimulation (25 μM for 24 hours), the level of cell viability in shSLC1A5 Huh7 cells was much lower than it was in shControl cells. Lirpoxstatin-1 could reverse the enhanced ferroptosis effect induced by knock-down of SLC1A5. (f) shSLC1A5 dramatically improved the liver tumor cells’ sensitivity to irradiation-induced LPO accumulation. RT: 8 Gy. AFGs: amino acid-ferroptosis genes; ferr-1: ferrostatin-1. .

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