GSK 650394 Inhibits Osteoclasts Differentiation and Prevents Bone Loss via Promoting the Activities of Antioxidant Enzymes In Vitro and In Vivo

Figure 1

The cytotoxicity of GSK at determined concentrations did not suppress the viability of osteoclast precursor cells. (a) The structure of GSK. (b–d) The viability of BMMs, HEPG2, and MC3T3 E1 treated with different levels of GSK for 12 hours, 24 hours, 48 hours, 72 hours, and 120 hours, as tested by CCK-8 assay. (e) Fluorescence microscopy images of BMMs, HEPG2, and MC3T3-E1 cells stained by LIVE/DEAD Kit after 3 days treatment with GSK. Calcine-AM (green) is used to stain live cells, while ethidium homodimer is used to stain dead cells (red color) scale bar: 50 μm. Data are presented as , (, ).

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