GSK 650394 Inhibits Osteoclasts Differentiation and Prevents Bone Loss via Promoting the Activities of Antioxidant Enzymes In Vitro and In Vivo

Figure 3

GSK regulates level of reactive oxygen species (ROS) and activities of antioxidant enzymes in vitro. (a) ROS levels in BMMs using DCF-DA. Scale bar: 20 μm. (b) Quantitative analysis of DCF fluorescence intensity. (c) Representative images protein expression of HO-1, GSR, and CAT. (d–f) Quantitative analysis of protein intensity of HO-1, GSR, and CAT relative to β-actin. (g–i) Antioxidant enzyme genes of BMMs treated with M-CSF and RANKL with/without different GSK concentrations (0, 1, 2, or 5 μM) for 5 days. (j–l) Antioxidant enzyme genes of BMMs treated with or without 5 μM GSK, for 1, 3, or 5 days, respectively. Data are expressed as the , . , , , versus -GSK group. Abbreviations: CAT (Cat): catalase; GSR (Gsr): glutathione-disulfide reductase; HO-1 (Homx1): heme oxygenase; ROS: reactive oxygen species; BMMs: bone marrow macrophages.

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