Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis

Figure 6

Harpagophytum procumbens (HP) ameliorates oxidative stress through attenuating iron deposition after lumbar spinal stenosis (LSS) induction. (a) Representative immunohistochemical images of ferritin (red) and iNOS (green) in spinal cord tissue at 1 week after LSS induction in rats of each group. White  μm, red  μm. (b) Quantitative analysis of iNOS intensity costained with ferritin in spinal cord tissue. (c–j) Gene expression from real-time PCR for oxidative stress-related genes; (c) iNOS and (d) Cox2, iron metabolism-related genes; (e) TFRC, (f) DMT1, (g) FTH1, (h) FTL, (i) CP, and (j) HAMP. Data are expressed as the . Significant differences are indicated as ^## and ^#### compared vs. the sham group, , , , and vs. the control group were analyzed via one-way ANOVA with Tukey’s post hoc test.

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