Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis

Table 1

Primer sequences used for real-time polymerase chain reaction analysis.

Gene	5–3	Primer sequence
TNF-α	Forward	CCGACTACGTGCTCCTCACC
	Reverse	CTCCAAAGTAGACCTGCCCG
IL-6	Forward	CCACCCACAACAGACCAGTA
	Reverse	GGAACTCCAGAAGACCAGAGC
IL-1β	Forward	TTGCTTCCAAGCCCTTGACT
	Reverse	GGTCGTCATCATCCCACGAG
IL-10	Forward	TAACTGCACCCACTTCCCAG
	Reverse	AGGCTTGGCAACCCAAGTAA
Arg1	Forward	GTCTCCAGATGCCTTTGCTTC
	Reverse	ATGAAATTCAGGGTGTGGGAAT
iNOS	Forward	ATGGCTTGCCCCTGGAAGTT
	Reverse	TGTTGGGCTGGGAATAGCAC
Cox2	Forward	CTCAGCCATGCAGCAAATCC
	Reverse	GGGTGGGCTTCAGCAGTAAT
TFRC	Forward	GGCTATGAGGAACCAGACCGCTACA
	Reverse	TGGACTTCGCAACACCAGGGC
DMT1	Forward	TGTCGCCTGTCCATTTGGCCG
	Reverse	TGGCGTGGCGGGGTTGAAAT
Fth1	Forward	TTGCAACTTCGTCGCTCCGCC
	Reverse	TGGCGCACTTGCGAGGGAGA
Nrf2	Forward	GATCTGTCAGCTACTCCCAG
	Reverse	GCAAGCGACTCATGGTCATC
Nf200	Forward	AACACCACTTAGATGGCGGG
	Reverse	ACGTGGAGCGTTCAGCAATA
Wnt3	Forward	CCAATTTGGTGGTCCCTGGC
	Reverse	TAATTGCGGCAGAAACGCAG
Wnt5a	Forward	GTTGAAGCCACAAGAGACAGC
	Reverse	AGAGCATGAGCCTTTTCGGT
Gapdh	Forward	CCCCCAATGTATCCGTTGTG
	Reverse	TAGCCCAGGATGCCCTTTAGT