Myostatin Deficiency Enhances Antioxidant Capacity of Bovine Muscle via the SMAD-AMPK-G6PD Pathway

Figure 6

MSTN affects the content of GSH through TGF-β-AMPK-G6PD in cattle muscle satellite cells. (a) Co-IP analysis of G6PD Ser, Thr phosphorylation in cattle muscle. (b) Co-IP analysis of G6PD tyrosine phosphorylation in cattle muscle. (c) AMPK activator (AICAR, 300 μM), inhibitor (dorsomorphin 2HCl, 5 μM), Smad activator (alantolactone, 120 μM), and Smad inhibitor (SIS3 HCL, 3 μM). WT muscle satellite cells were treated with for 3 h. The influence of Western blot on Smad2+3, p-AMPKα1+α2, and G6PD. (d) WT muscle satellite cells were treated with AMPK activator and Smad inhibitor for 3 h. Co-IP analysis of the tyrosine phosphorine of G6PD in cattle muscle. (e) WT muscle satellite cells were treated with AMPK activator and Smad inhibitor for 3 h to determine the content of GSH. MT: MSTN knockout cattle group; WT: wild-type cattle group. Data presented are . One-way ANOVA with post hoc LSD multiple comparison test. , .

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