Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury

Figure 2

CPB promoted CIRP secretion and aggravated renal injury. (a) H&E staining showing more dilated renal tubules and tube casts in the CPB group compared to the sham group and CPB+C23 group. (b, c) Expression levels of Scr at 0 and 4 hours after CPB. (d, e) Expression levels of BUN at 0 and 4 hours after CPB. (f, g) Expression levels of CIRP at 0 and 4 hours after CPB. (h, i) TUNEL staining indicating the increased number of apoptotic cells in the renal tissue after CPB; C23 administration effectively reduced apoptosis (magnification 200x). (j, k) The mRNA levels of renal injury markers KIM-1 and NGAL in renal tissues. CIRP: cold-inducible RNA-binding protein; CPB: cardiopulmonary bypass; Scr: serum creatinine; BUN: blood urea nitrogen. vs. the sham group; ^# vs. CPB. (a, h, top) Scale bar: 200 μm and (a, bottom) scale bar: 100 μm.

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