Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury

Figure 5

CIRP mediated oxidative stress and mitochondrial dynamics disorder in HK-2 cells. (a) Renal DHE staining (red) and counterstaining DAPI (blue), magnification 400x. (b) Renal DHE fluorescence intensity in each group. (c–e) Expression of inflammatory factors IL-6, IL-1β, and TNF-α in the medium. (f, g) Flow cytometry analysis of HK-2 cell apoptosis. (h, i) The mRNA levels of KIM-1 and NGAL. (j) Western blot analysis of NADPH oxidase and mitochondrial proteins, rhCIRP, and recombinant human CIRP protein. DHE: dihydroethidium; NADPH: nicotinamide adenine dinucleotide phosphate. vs. control; ^# vs. high rhCIRP. (a) Scale bars: 100 μm.

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