The Activation of AMPK/NRF2 Pathway in Lung Epithelial Cells Is Involved in the Protective Effects of Kinsenoside on Lipopolysaccharide-Induced Acute Lung Injury

Figure 6

AMPKα deficiency abolished the protective effects of Kin (100 mg/kg) in mice challenged with LPS. (a) Representative Western blots of AMPKα in wild-type mice and AMPKα knockout mice. (b) Representative images of H&E staining of lung tissues from AMPKα knockout mice and wild-type mice (), scale bars, 100 μm. (c) The lung wet-to-dry ratio (). (d, e) The mRNA levels of IL-1β and Tnf-α in lung tissues (). (f) SOD activity in lung tissues (). (g–k) The mRNA levels of Drp1, Mfn1, Mfn2, Opa1, and Fis in lung tissues (). (l) Representative Western blots and quantitative results of NRF2 (). The data are expressed as the . (versus the indicated groups); NS: no statistical significance.

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