Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

Figure 6

MSCs-Exo inhibit hippocampus ferroptosis in dNCR mice. The mice were randomly divided into 4 groups: Sham group, dNCR group, dNCR+MSCs-Exo group, and dNCR+Fer-1 group. (a) TEM was employed to detect the ultrastructure of hippocampus in aged mice ( μm). (b, c) ROS (red fluorescent signal) were detected using DHE staining ( μm). (d) The GSH level by GSH Colorimetric Assay Kit. (e) The MDA level by MDA Colorimetric Assay Kit. (f) The Fe²⁺ level by Ferrous Iron Colorimetric Assay Kit. (g–j) GPX4, P53, and SLC7A11 expressions in each group were determined by Western blot. gprot: gram protein; mgprot: milligram protein. Data are expressed as (/group). and , Sham vs. dNCR; ^##, dNCR vs. dNCR+ MSCs-Exo; ^@@, dNCR vs. dNCR+Fer-1. Fer-1: ferrostatin-1, a ferroptosis inhibitor.

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