Research Article
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway
Figure 9
MSCs-Exo alleviated hippocampus ferroptosis of dNCR mice in a SIRT1-dependent manner. The mice were randomly divided into 5 groups: Sham group, dNCR group, dNCR+MSCs-Exo group, dNCR+MSCs-Exo + EX-527 group, and dNCR+EX-527 group. (a) TEM was employed to detect the ultrastructure of hippocampus in aged mice ( μm). (b, c) ROS (red fluorescent signal) were detected using DHE staining ( μm). (d) The GSH level by GSH Colorimetric Assay Kit. (e) The MDA level by MDA Colorimetric Assay Kit. (f) The Fe2+ level by Ferrous Iron Colorimetric Assay Kit. (g–j) GPX4, P53, and SLC7A11 expressions in each group were determined by Western blot. (k–m) Nrf2 and HO-1 expressions in each group were determined by Western blot. gprot: gram protein; mgprot: milligram protein. Data are expressed as (/group). , , and , Sham vs. dNCR or Sham vs. dNCR+EX-527; #, ##, ###, dNCR vs. dNCR+MSCs-Exo; $, $$, and $$$, dNCR+MSCs-Exo vs. dNCR+MSCs-Exo + EX-527. EX-527: a specific SIRT1 inhibitor.
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