Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy

Figure 5

Signal pathway of FGN upregulating SOD expression in sBregs. LPMCs were isolated from OVA-primed mouse intestine and cultured in the conditions denoted below (e). (a–c) Immunoblots show (a) STAT3/pSTAT3, (b) p38/p-p38, and (c) NF-κB/pNF-κB in sBregs. (d) Boxplots and (e) immunoblots show SOD levels in sBregs. FGN: 1 μg/ml; OVA: 5 μg/ml; BSA: 5 μg/ml; CPT: Cryptotanshinone (a STAT3 inhibitor, 5 μM); SB: SP203580 (a p38 inhibitor, 10 μM); Indi: indicaxanthin (an NF-κB inhibitor, 20 μM). The data of boxplots are presented as median (IQR). The data represent 3 independent experiments.

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