Antioxidant Activity of Valeriana fauriei Protects against Dexamethasone-Induced Muscle Atrophy

Figure 3

VF prevents DEX-induced myotube atrophy in C2C12 cells. (a) Immunofluorescence staining for MHC (green) and counterstaining with DAPI (blue) in C2C12 myotubes. (b) Fusion index (%) was calculated. (c) Myotube diameter (μm) was measured. (d) The distribution (%) of myotube diameter (μm). (e) Protein expression of MHC isoforms (total MHC, MHC1, MHC2A, and MHC2B) in VF-treated C2C12 myotubes was measured by western blot analysis. (f–h) The mRNA expression of muscle atrophy markers, including Atrogin-1 (f), Murf1 (g), and Mstn (h), was measured by qRT-PCR. The data are presented as the . Statistical significance was determined by one-way ANOVA. , versus the nontreated group.

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